به نام خدا
Title: DNA extraction method with improved efficiency and specificity using DNA methyltransferase and click chemistry
Authors: Alexander B Artyukhin , YounHi Woo
Abstract: In an attempt to develop an alternative method to extract DNA from complex samples with much improved sensitivity and efficiency, here we report a proof-of-concept work for a new DNA extraction method using DNA methyltransferase (Mtase) and click chemistry. According to our preliminary data, the method has improved the current methods by (i) employing a DNA-specific enzyme, TaqI DNA Mtase, for improved selectivity, and by (ii) capturing the DNA through covalent bond to the functionalized surface, enabling a broad range of treatments yielding the final sample DNA with minimal loss and higher purity such that it will be highly compatible with downstream analyses. By employing Mtase, a highly DNA specific and efficient enzyme, and click chemistry, we demonstrated that as little as 0.1 fg of k-DNA (close to copy number 1) was captured on silica (Si)-based beads by forming a covalent bond between an azide group on the surface and the propargyl moiety on the DNA. This method holds promise in versatile applications where extraction of minute amounts of DNA plays critical roles such as basic and applied molecular biology research, bioforensic and biosecurity sciences, and state-of-the-art detection methods.
Publish Year: 2012
Published in: Analytical Biochemistry - Journal of Science Direct
Number of Pages: 6
موضوع: شیمی، بیوشیمی
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: The Application of Coal Cleaning Detection System based on Embedded Real-time Image Processing
Authors: Qian Mu ,Jixian Dong
Abstract: This paper introduces a high-speed image processing of embedded systems based on FPGA and DSP collaboration used in coal preparation detection technology. The system uses a modular design, including a real-time image acquisition module, DSP image processing module, and peripheral interface module. The image processing required doing the following arithmetic operations, including image smoothing to enhance, edge detection, region segmentation, gray-scale analysis, pattern recognition. After completion of the above algorithm, the system determines the image feature of coal and gangue, particularly their gray scale values and the center of gravity. By analysis of the characteristics of the image, the system identifies the gangue. The image processing is conducive to the follow-up to discharge refuse treatment.
Publish Year: 2013
Published in: ICMTMA - IEEE
Number of Pages: 3
موضوع: پردازش تصویر
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حامی دانش بومی ایرانیان
به نام خدا
Title: Business Process based Initial Modeling at Software Development
Authors: J Tick
Abstract: The fundamental problem of high-level quality software development is the elaboration of well-designed and correct model of the developing system. The most critical phase is the “birth” of the model, the specification of the initial model. This paper describes the method to use the already existing Business Process Model to set up the initial model for the software to be developed. Especially Business Process Management systems can be well described by business processes using workflow. This paper makes recommendations how to incorporate workflow in the development process. The basic principle of the method to elaborate the initial model is that using the usually already existing workflow an UML action diagram can be worked out with the help of mapping that can be a useful additional source for further development processes.
Publish Year: 2013
Published in: SAMI - IEEE
Number of Pages: 4
موضوع: توسعه نرم افزار، مهندسی نرم افزار
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: Qcompiler: Quantum compilation with the CSD method
Authors: YG Chen, JB Wang
Abstract: In this paper, we present a general quantum computation compiler, which maps any given quantum algorithm to a quantum circuit consisting a sequential set of elementary quantum logic gates based on recursive cosine sine decomposition. The resulting quantum circuit diagram is provided by directly linking the package output written in LaTeX to Qcircuit.tex We illustrate the use of the Qcompiler package through various examples with full details of the derived quantum circuits. Besides its accuracy, generality and simplicity, Qcompiler produces quantum circuits with significantly reduced number of gates when the systems under study have a high degree of symmetry.
Publish Year: 2013
Published in: Computer Physics Communications - Science Direct
Number of Pages: 13
موضوع: رایانش کوانتوم ، فیزیک
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: Traffic Information Based Power Saving Mechanism
Authors: Ju No Han, Seong Gon Choi
Abstract: This paper proposes the standby power saving mechanism that the user can terminate home network appliance (Le. Personal Computer) power and standby power at the remote site. Generally, for the power management of the home appliance, it has to be put through the physical contact. In the proposed mechanism, the smartphone is offered the power on/off state information of the home network appliance on a real-time basis. The user can transmit the power-termination order to the home network appliance through the smartphone. If the home gateway receives the power-termination order from the smartphone, the power-termination order is sent to the home network appliance and the standby power is blocked. Its power and standby power can be blocked through the method to be proposed even through the user is not physically contacted with the home appliance. That is, the proposed mechanism gives convenience to the user in electric power management aspect. In addition, the power saving can be done, because reducing the unnecessary operation time and blocking the standby power of the home network appliance.
Publish Year: 2013
Published in: ICACT - IEEE
Number of Pages: 4
موضوع: مهندسی برق – الکترونیک
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان